Fall 2011
Biology
4480/5480, Cytology and Microtechnique
Lecture (Dr. Joseph R Koke) - 9:30 - 10;50 T- Th 435 SUPPLE HALL
Lab (Ms. Sarah J. Kane) - 3:30 to 4:45 Tuesday (and more!) 435 SUPPLE HALL
Last updated, August 24, 2011 .
The direct (outside of TRACS) URL for this page is http://www.jkoke.net/C&M/index.html

The objectives of this course are (1) to provide the theoretical background and practical information necessary to perform modern microscopy, (2) provide hands-on training sufficient to qualify students completing this course (with a B or better) as "microscopy technicians" in a clinical or research setting (academic or industry), and (3) to train students in biological ultrastructure sufficiently to accurately interpret the images they learn to create.

Please check this syllabus and your 4480/5480 TRACS frequently throughout the semester. Note that the lecture notes and lab manuals can be downloaded (PDF) from the TRACS Cytology and Microtechnique Resources - Course Documents folder for this site. A lap top computer (preferred) or notepad (e.g., Apple iPad), while not required is highly recommended for this course. You may download the lecture PDFs from TRACS to your lap-top, bring it to lecture and take notes electronically.

If requested, B&W paper copies of these notes will be provided in lecture - however, much of microscopy depends on color. If you want color copies, print those at your own expense.


Date Lecture Topic Laboratory (first meeting is Tuesday, September 6)
August 25 - 30 Introduction, History of Microscopy, Course Overview SEE TRACS RESOURCES (for this course) for protocols and manuals specific to the Biology IMF (Intergrated Microscopy Facility). You will need to bring them with you - either electronically or on paper - when receiving laboratory instruction and when using the equipment to which the manual/handout applies. If you are working in a group, only 1 manual/group is necessary.
September 1

no class

.

Sept. 6 - Oct. 18.
  • Fixation, Tissue Processing
  • Optics, Microscopes
  • Microscopy, light, electron, atomic force and STM
  • Quantitative Techniques
  • Digital Microscopy and image analysis

September 6. Lab Organizational meeting. LAB SYLLABUS

  • Short weekly meetings (3:30 on Tuesdays) will be in 435 SUPP (See Lab Syllabus). Attendance is required! Most microscopy will be done in the Biology IMF Suite (171)
  • BIO-IMF standard protocols
  • BIO-IMF microtomy
  • BIO IMF TEM operation
  • BIO IMF GATAN digital camera MANUAL
October 18 Mid-Semester Exam Take home exam - due 9:00 Tuesday Nov. 1
Oct. 20 - Nov. 1 No lecture - independent lab instruction,
Nov. 1 -
December 1
  • Cytology 1 - Ultrastructure of Prokaryotes, Fungi, Plants
  • Cytology 2 - The Nucleus
  • Cytology 3 - Cytoskeleton
  • Cytology 4 - Junctions
  • Cytology 5 - Membranes, ER, and Golgi
  • Cytology 6 - Organelles
  • All lab work must be complete by the end of the day Wednesday, Nov. 30.
  • Supple 437 and 435 will be available Dec. 1-4 to facilitate poster preparation.
  • Posters must be posted by Sunday 11:59 p.m., Dec. 4.
  • Poster session(s) will be scheduled Monday, Dec. 5.
TBA Final Exam, Tuesday, December 13, 8 - 10:30, 153 SUPP Hall. The final exam is a practical exam and you must be present to take it.

Recommended Resources
Author
Electron Microscopy (book) Bazolla and Russell
Microscopy, Immunohistochemistry, and Antigen Retrieval Methods (book) M. A. Hayat
Handbook of biological confocal microscopy (book) J. B. Pawley
Cell biological applications of confocal microscopy (book) B. Matsumoto
Methods for TEM, Methods for LM (Web) IHC World
Molecular Expressions - Optical Microscopy Primer (Web) Florida State U.
Nikon Microscopy U (Web) Nikon Web
Olympus Microscopy Resource Center (Web) Olympus Web

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