Fall 2011
Biology 4480/5480, Cytology and Microtechnique
Lecture (Dr. Joseph R Koke) - 9:30 - 10;50 T- Th 435
SUPPLE HALL
Lab (Ms. Sarah J. Kane) - 3:30 to 4:45 Tuesday (and more!) 435 SUPPLE HALL
Last updated
The direct (outside of TRACS) URL for this page is http://www.jkoke.net/C&M/index.html
The objectives of this course are (1) to provide the theoretical background and practical information necessary to perform modern microscopy, (2) provide hands-on training sufficient to qualify students completing this course (with a B or better) as "microscopy technicians" in a clinical or research setting (academic or industry), and (3) to train students in biological ultrastructure sufficiently to accurately interpret the images they learn to create.
Please check this syllabus and your 4480/5480 TRACS frequently throughout the semester. Note that the lecture notes and lab manuals can be downloaded (PDF) from the TRACS Cytology and Microtechnique Resources - Course Documents folder for this site. A lap top computer (preferred) or notepad (e.g., Apple iPad), while not required is highly recommended for this course. You may download the lecture PDFs from TRACS to your lap-top, bring it to lecture and take notes electronically.
If requested, B&W paper copies of these notes will be provided in lecture - however, much of microscopy depends on color. If you want color copies, print those at your own expense.
| Date | Lecture Topic | Laboratory (first meeting is Tuesday, September 6) |
|---|---|---|
| August 25 - 30 | Introduction, History of Microscopy, Course Overview | SEE TRACS RESOURCES (for this course) for protocols and manuals specific to the Biology IMF (Intergrated Microscopy Facility). You will need to bring them with you - either electronically or on paper - when receiving laboratory instruction and when using the equipment to which the manual/handout applies. If you are working in a group, only 1 manual/group is necessary. |
| September 1 |
no class |
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| Sept. 6 - Oct. 18. |
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September 6. Lab Organizational meeting. LAB SYLLABUS
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| October 18 | Mid-Semester Exam | Take home exam - due 9:00 Tuesday Nov. 1 |
| Oct. 20 - Nov. 1 | No lecture - independent lab instruction, | |
| Nov. 1 - December 1 |
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| TBA | Final Exam, Tuesday, December 13, 8 - 10:30, 153 SUPP Hall. The final exam is a practical exam and you must be present to take it. | |
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| Electron Microscopy (book) | Bazolla and Russell |
| Microscopy, Immunohistochemistry, and Antigen Retrieval Methods (book) | M. A. Hayat |
| Handbook of biological confocal microscopy (book) | J. B. Pawley |
| Cell biological applications of confocal microscopy (book) | B. Matsumoto |
| Methods for TEM, Methods for LM (Web) | IHC World |
| Molecular Expressions - Optical Microscopy Primer (Web) | Florida State U. |
| Nikon Microscopy U (Web) | Nikon Web |
| Olympus Microscopy Resource Center (Web) | Olympus Web |
All rights reserved. No text or images may be reproduced without the copyright owners' express written permission. Intellectual Property Statement: My lectures and my examination questions and other course materials are protected by copyright law. They are my own original expression and are properly recorded to bring them under the protection of the U.S. copyright laws. You are authorized to take notes in class and to be provided answers to examination questions, thereby creating derivative works from my lectures and examinations. However, this authorization extends only to making one set of notes or answers for your own personal use and no other use. You are not authorized to record my lectures, to provide notes or examination questions to anyone else, or to make any commercial use of them without my prior written consent.